مشخصات پژوهش

In this work, the separation and purification of 1,5-Di-O-caffeoylquinic acid (1,5 DCQA) was carried out via columnchromatography (ethyl acetate/n-hexane with volume ratio 20/80) from Artichoke (Cynara scolymusL.) head ethanolic extractand characterized with IR, LC/MS, and UV/visible. The electrochemical approach has been used to analyze and detect electroactivecompounds in ethanolic extract. Differential pulse voltammetry results indicated that a bioactive and electroactive molecule(1,5-Di-O-caffeoylquinic acid) is the major constituent in the Artichoke ethanolic extract. The analytical parameters are calculatedvia calibration curve and obtained 7.8μM and 9.0μM–1.8 mM for LOD and linear range, respectively. The theoretical andexperimental biological assessment has been carried out with molecular docking, 2,2-diphenyl-1-picrylhydrazyl (DPPH), andin vitro antibacterial susceptibility assay. The adsorption peak of DPPH is thoroughly deleted after the addition of Artichokeextract; therefore, its antioxidant activity is achieved approximately 100%. The inhibition zone surrounding the wells indicated thatrelevant extract has inhibitory function againstE. coli(19 mm),S. enterica(21 mm),B. cereus(20 mm), andS. aureus(17 mm)bacterial. The binding affinity values showed that1,5 DCQAhas an inhibitory effect against ROS generation enzymes andbacterial enzymes